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![Cyclin D1 Antibody [DCS-6]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/574940/810996/porsci_lo__79508.1648973713__42722.1649091764.png?c=2 "Cyclin D1 Antibody [DCS-6]")
![Cyclin D1 Antibody [DCS-6]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/574940/810996/porsci_lo__79508.1648973713__42722.1649091764.png?c=2 "Cyclin D1 Antibody [DCS-6]")
Product Description
Cyclin D1 Antibody [DCS-6] | 33-068 | ProSci
Host: Mouse
Reactivity: Human, Monkey, Mouse, Rat
Homology: N/A
Immunogen: Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
Research Area: Cancer, Cell Cycle
Tested Application: WB, Flow, IHC, IF
Application: Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: DCS-6
Isotype: IgG2a, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody recognizes a protein of 36kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
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![Cyclin D1 Antibody [BCLD1-1]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575685/812775/porsci_lo__79508.1648973713__48394.1649092008.png?c=2 "Cyclin D1 Antibody [BCLD1-1]")
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