Product Description
Cytokeratin5 Antibody [3E2F1] | 32-142 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant CK5-Trx-His expressed in E. Coli strain BL21 (DE3) .
Research Area: Other
Tested Application: E, WB, IHC
Application: Western Blot:1:500 - 1:2, 000
IHC (P) :1:500 - 1:2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 3E2F1
Isotype: IgG1
Conjugate: Unconjugated
Physical State: N/A
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: Cytokeratin 5 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: 58 kDa cytokeratin, CK-5, K5, CK5, DDD, DDD1, EBS2, KRT5A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides) . The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB) . Defects in KRT5 are a cause of epidermolysis bullosa simplex.