Product Description
Dynamin-1 Antibody [3G4B6] | 32-146 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant Dynamin-1 expressed in E. Coli strain BL21 (DE3) .
Research Area: Neuroscience
Tested Application: E, WB, IHC
Application: Western Blot:1:500 - 1:2, 000
IHC (P) :1:500 - 1:2, 000
IHC (F) :1:500 - 1:2, 000
ELISA:Propose dilution 1:10, 000
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 3G4B6
Isotype: IgG2a
Conjugate: Unconjugated
Physical State: N/A
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: Dynamin-1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: DNMDNM
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Dynamin-1 (Dyn1) , with 864-amino acid protein (about 95 kDa) , belongs to the dynamin family. Dynamin-1 (neuron-specific) , dynamin-2 (ubiquitously expressed) , and dynamin-3 (expressed only in the testis, brain, and lung) , constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin-1 is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin-1 is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin-1 is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin-1 gene expression is induced during neuronal differentiation.