Product Description
EphA1 Antibody [5D2A4 / 5D2A10] | 32-150 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant EphA1 expressed in E. Coli strain BL21 (DE3) .
Research Area: Neuroscience, Growth Factors
Tested Application: WB, IHC
Application: Western Blot:1:500 - 1:2, 000
IHC (P) :1:500 - 1:2, 000
IHC (F) :1:500 - 1:2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein A affinity chromatography.
Clonality: Monoclonal
Clone: 5D2A4 / 5D2A10
Isotype: IgG1
Conjugate: Unconjugated
Physical State: N/A
Buffer: Purified antibody in PBS containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: EphA1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: EPH, EPHT, EPHT1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: EPH receptor A1 (EphA1) , with 976-amino acid protein (about 107 kDa) , belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date and their ligands, the ephrins, can be subdivided into two major subclasses, ephrin-A and ephrin-B. Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior, particularly in the nervous system. They have been implicated in various developmental processes, including axonal guidance, angiogenesis, morphogenesis and carcinogenesis.