Euro
USD
British Pound
NULL
![G-CSF Antibody [CSF3/900]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575196/811604/porsci_lo__79508.1648973713__67746.1649091854.png?c=2 "G-CSF Antibody [CSF3/900]")
![G-CSF Antibody [CSF3/900]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575196/811604/porsci_lo__79508.1648973713__67746.1649091854.png?c=2 "G-CSF Antibody [CSF3/900]")
Product Description
G-CSF Antibody [CSF3/900] | 33-340 | ProSci
Host: Mouse
Reactivity: Human, Macaque
Homology: N/A
Immunogen: Human recombinant full-length protein was used as the immunogen for the G-CSF antibody.
Research Area: Cancer, Immunology, Signal Transduction
Tested Application: Flow, IF, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the G-CSF antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: CSF3/900
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This mAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73% sequence identity at the amino acid level.
![G-CSF Antibody [CSF3/900]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575196/811604/porsci_lo__79508.1648973713__67746.1649091854.png?c=2 "G-CSF Antibody [CSF3/900]")
