Product Description
GAPDH Antibody [2D4A7] | 32-159 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3) .
Research Area: Neuroscience
Tested Application: E, WB, IHC, IF
Application: Western Blot:1:500 - 1:2, 000
IHC (P/F) :1:200 - 1:1, 000
IF:1:200 - 1:1, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 2D4A7
Isotype: IgG1
Conjugate: Unconjugated
Physical State: N/A
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: GAPDH monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: GAPD, CDABP0047, OK/SW-cl.12, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, G3PD, GAPD, HEL-S-162eP
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer’s and Huntington’s disease.