Product Description
Glycophorin A Antibody [GYPA/280] | 33-385 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.
Research Area: Homeostasis
Tested Application: WB, Flow, IF, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the Glycophorin A antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: GYPA/280
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: GYPA, glycophorin A (includes MN blood group) , HGNC:4702, GPA, GPErik, GPSAT, HGpMiV, HGpMiX, HGpMiXI, HGpSta (C) , MN, MNS, Mi.V glycoprotein (24 AA) , erythroid-lineage-specific membrane sialoglycoprotein, glycophorin A, glycophorin Er
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA) . It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB) , which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.