Product Description
HCG-beta Antibody [HCGb/211] | 33-269 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Purified HCG-beta protein was used as the immunogen for this antibody.
Research Area: Other
Tested Application: WB, IHC-Fr, E
Application: ELISA: use BSA-free format (1)
IHC (Frozen) : 0.5-1 ug/ml for 30 min at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.
1. Clone HCGb/211 (recommended capture) will pair with clones HCGb/459 and HCGb/54 (recommended detect) by ELISA.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: HCGb/211
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: CGB, CGB3, CG-beta, Choriogonadotropin beta chain, HCGB, hCG Beta
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody reacts with a protein of 22kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH) , follicle stimulating hormone (FSH) , and luteinizing hormone (LH) . HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90% and 60% of cases respectively; 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.