Product Description
HMW Cytokeratin Antibody [34BE12] | 33-239 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.
Research Area: Signal Transduction
Tested Application: WB, IHC, Flow, IF
Application: Flow Cytometry: 0.5-1ug/10e6 cells
IF: 0.5-1.0 ug/ml
WB: 0.5-1.0 ug/ml
IHC (FFPE) : 0.5-1.0 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: 34BE12
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, CK-1, Hair alpha protein, Keratin-1, K1, Type-II keratin Kb1, KRT1, KRTA
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.