Product Description
IL-33 Antibody [12H6D12] | PM-5041 | ProSci
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Mouse monoclonal IL-33 antibody was raised against the recombinant IL-33 protein.
Research Area: Immunology
Tested Application: E, WB, IHC-P
Application: IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 95-106 - IL-33 Recombinant Protein
Positive Control 2: Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: IL-33 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Clonality: Monoclonal
Clone: 12H6D12
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: IL-33 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: IL-33 monoclonal antibody can be stored at -20˚C, stable for one year.
Alternate Name: IL-33 Antibody [12H6D12] : DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: IL-33 Monoclonal Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1αβ, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP) . Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.