Product Description
IRAK Antibody [8F1A7] | PM-5317 | ProSci
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Recombinant protein corresponding to amino acids 618 - 712 of human IRAK.
Research Area: Signal Transduction, Innate Immunity,
Tested Application: E, WB, IHC-P
Application: IRAK antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 95-112 - IRAK Recombinant Protein
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: IRAK Monoclonal Antibody is Protein G purified.
Clonality: Monoclonal
Clone: 8F1A7
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: IRAK monoclonal antibody can be stored at -20˚C, stable for one year.
Alternate Name: IRAK Antibody [8F1A7] : IRAK, pelle
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: IRAK Monoclonal Antibody: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) .