Product Description
Ku70 + Ku80 Antibody [KU729] | 33-138 | ProSci
Host: Mouse
Reactivity: Human, Primate
Homology: N/A
Immunogen: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
Research Area: Cancer
Tested Application: ICC, Flow, IF
Application: Flow cytometry: 0.5-1ug/10e6 cells
IF: 0.5-1.0 ug/ml
Immunocytochemistry (Acetone-fixed cells) : 0.25-0.5 ug/ml for 30 minutes at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
Specificiy: Does not react with mouse, rat, chicken
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G purified monoclonal antibody
Clonality: Monoclonal
Clone: KU729
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: X-ray repair cross-complementing protein 6, 364-, 4299-, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Ku70, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 6, XRCC6, G22P1
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80) , identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.