Product Description
LIMK1 Antibody [G0G9] | 13-200 | ProSci
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: A synthetic peptide of human LIMK1
Research Area: Cell Cycle, Immunology, Neuroscience, Signal Transduction
Tested Application: WB
Application: WB: 1:500 - 1:1000
Specificiy: N/A
Positive Control 1: HeLa
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 75kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Monoclonal
Clone: [G0G9]
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, pH7.3.
Concentration: N/A
Storage Condition: Store at 4˚C. Avoid freeze / thaw cycles.
Alternate Name: LIMK1, LIM domain kinase 1, Lim kinase 1, LIMK-1, LIM kinase, LIMK
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.