Product Description
Lin28 Antibody [1G9H9] | PM-6143 | ProSci
Host: Mouse
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (87%) , Chicken: (79%)
Immunogen: Mouse monoclonal Lin28 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Lin28.
The immunogen is located within the last 50 amino acids of Lin28.
Research Area: Stem Cell
Tested Application: E, WB
Application: Lin28 antibody can be used for detection of Lin28 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificiy: At least two isoforms of Lin28 are known to exist; this antibody will detect both. Lin28 antibody will not cross-react with Lin28 Homolog B.
Positive Control 1: Cat. No. 1207 - Raji Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 23 kDa
Validation: N/A
Isoform: N/A
Purification: Lin28 Monoclonal Antibody is affinity chromatography purified via peptide column.
Clonality: Monoclonal
Clone: 1G9H9
Isotype: IgG2b
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Lin28 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Lin28 Monoclonal antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Lin28 Antibody [1G9H9] : CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Lin28 Monoclonal Antibody: Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.