Product Description
MCP-1 Antibody [2.2-4H5-01A11] | XP-5732-M | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hMCP-1/MCAF as the immunizing antigen.
Research Area: Chemokines & Cytokines
Tested Application: E, WB
Application: ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human MCP-1 when used with our biotinylated antigen affinity purified anti-human MCP-1 (XP-5218Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: This IgG1/K antibody was purified from ascites fluid on a Protein A affinity column.
Clonality: Monoclonal
Clone: 2.2-4H5-01A11
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: MCP-1 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Alternate Name: MCP1, SCYA2, C-C motif chemokine 2, HC11, MCAF, MCP-1, GDCF-2, SMC-CF, HSMCR30
User Note: Centrifuge vial prior to opening.
BACKGROUND: N/A