Product Description
MDA5 Antibody [Hely-1] | 36-114 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human MDA5 (aa 78-555) .
Research Area: Immunology, Innate Immunity
Tested Application: E, IP, WB
Application: Immunoprecipitation: (1:100) . Western Blot: (1:1‘000) .
Specificiy: Recognizes human MDA5.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: >95% (SDS-PAGE)
Clonality: Monoclonal
Clone: Hely-1
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.
Alternate Name: Helicard; Melanoma Differentiation-associated Gene 5
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation.