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![Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575101/811390/porsci_lo__79508.1648973713__10114.1649091814.png?c=2 "Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]")
![Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575101/811390/porsci_lo__79508.1648973713__10114.1649091814.png?c=2 "Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]")
Product Description
Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11] | 33-241 | ProSci
Host: Mouse
Reactivity: Human, Bovine, Rat, Mouse, Guinea pig, Frog, Goat, Marmoset, Pig
Homology: N/A
Immunogen: A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
Research Area: Signal Transduction
Tested Application: WB, Flow, IHC, IF
Application: Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: C11
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
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