Product Description
PPAR alpha Antibody [3D2G6] | 13-483 | ProSci
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Recombinant protein of human PPAR alpha
Research Area: Cancer, Signal Transduction, Transcription
Tested Application: WB, IHC, IF, Flow
Application: WB: 1:500 - 1:1000
IHC: 1:10 - 1:25
IF: 1:10 - 1:25
Flow: 1:10 - 1:25
Specificiy: N/A
Positive Control 1: HeLa
Positive Control 2: Jurkat
Positive Control 3: NIH/3T3
Positive Control 4: LO2
Positive Control 5: Mouse liver
Positive Control 6: Mouse kidney
Molecular Weight: Observed: 52kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Monoclonal
Clone: [3D2G6]
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, pH7.3.
Concentration: N/A
Storage Condition: Store at 4˚C. Avoid freeze / thaw cycles.
Alternate Name: PPARA, PPAR, NR1C1, hPPAR, PPARalpha
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.