Product Description
Rab25 Antibody [3F12F3] | 32-208 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3) .
Research Area: Signal Transduction
Tested Application: E, WB, IHC
Application: Western Blot:1:500 - 1:2, 000
IHC (P) :1:500 - 1:2, 000
IHC (F) :1:500 - 1:2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide.
Clonality: Monoclonal
Clone: 3F12F3
Isotype: IgG2a
Conjugate: Unconjugated
Physical State: N/A
Buffer: Purified antibody in PBS containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: Rab25 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: CATX8, CATX-8CATX-8, RAB11C
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.