Product Description
RSK1 Antibody [10B1D7A9 / 10B1D7E12] | 32-212 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant RSK1 expressed in E. Coli strain BL21 (DE3) .
Research Area: Signal Transduction
Tested Application: E, WB, IHC
Application: Western Blot:1:500 - 1:2, 000
IHC (P) :1:500 - 1:2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 10B1D7A9 / 10B1D7E12
Isotype: IgG2a
Conjugate: Unconjugated
Physical State: N/A
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: RSK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: MAPKAPK1A, RSK1, 90 kDa ribosomal protein S6 kinase 1, S6K-alpha-1, RSK, HU-1, MAPKAPK1A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Rsk1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine / threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK proteins possess two separate kinase domains, the C terminal kinase domain and the N terminal kinase domain, separated by a linker region containing a hydrophobic motif. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation.