Product Description
TGF alpha Antibody [1E8-G6] | 33-212 | ProSci
Host: Mouse
Reactivity: Human, Rabbit, Zebrafish
Homology: N/A
Immunogen: A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.
Research Area: Growth Factors, Antibody Pairs, Functional Assays
Tested Application: Flow, IHC, IF
Application: Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (Formalin-paraffin) : 1-2 ug/ml for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: PEG precipitation
Clonality: Monoclonal
Clone: 1E8-G6
Isotype: IgM, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.