Product Description
TLR9 Antibody [TR9541] | 33-002 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: A recombinant protein fragment from the N-terminal portion of human TLR9 was used as the immunogen for this antibody.
Research Area: Signal Transduction, Innate Immunity
Tested Application: WB, Flow, IHC
Application: Western blot: 2-4 ug/ml
Flow Cytometry: 1ug/10e6 cells
Immunohistochemistry (Paraffin) : 3-5 ug/ml (1)
1. For optimal TLR9 staining, formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G Affinity Chromatography
Clonality: Monoclonal
Clone: TR9541
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.5 mg/mL
Storage Condition: Aliquot and Store at -20˚C. Avoid freez-thaw cycles.
Alternate Name: TLR9 Antibody: CD289, Toll-like receptor 9
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: The mammalian immune system is composed of two components: innate immunity and adaptive immunity. Toll-like receptors (TLRs) are an essential part of the innate immune system. Through recognition of pathogen-associated molecular patterns (PAMPs) , TLRs mount an immune defense against pathogens. There are 10 mammalian TLRs (TLR1-10) . Unmethylated CpG sequences in bacterial genomes and viral DNA molecules activates TLR9, leading to the inflammatory response.