Product Description
TNF-a Antibody [2.1_4E10-1H11] | XP-5711-M | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hTNF-alpha as the immunizing antigen.
Research Area: Immunology, Chemokines & Cytokines, Signal Transduction, Growth Factors, Functional Assays
Tested Application: E, WB, Neut
Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.5 ng/mL) , a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human TNF-α when used with our biotinylated antigen affinity purified anti-human TNF-α (XP-5282Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 2.1_4E10-1H11
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: TNF-α lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Alternate Name: tumor necrosis factor alpha, TNFA, TNFSF2, Tumor necrosis factor, Cachectin, TNF-a,
User Note: Centrifuge vial prior to opening.
BACKGROUND: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.