Product Description
TYRO3 Antibody [1C10E8 / 9A3F2] | 32-233 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant TYRO3 expressed in E. Coli strain BL21 (DE3) .
Research Area: Signal Transduction, Neuroscience
Tested Application: E, WB, IF
Application: Western Blot:1:500 - 1:2, 000
IF:1:50 - 1:200
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 1C10E8 / 9A3F2
Isotype: IgG1
Conjugate: Unconjugated
Physical State: N/A
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: TYRO3 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: BYK, DTK, RSE, SKY, TIF, Tyrosine-protein kinase BYKBYK, Dtk, Sky, Tif
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Tyrosine-protein kinase (TYRO3) belongs to the Tyr protein kinase family (AXL/UFO subfamily) . The UFO family of receptor tyrosine kinases is comprised of subfamily members Rse (also referred to as Tyro3 or Sky) and UFO (also called Tyro7 or Axl) . Two distinct isoforms of Rse, designated Brt and Etk-2, have been described. Brt differs from Rse at its C-terminus, but more importantly lacks the N-terminal 31 amino acid signal peptide sequence present in Rse, which is replaced by a 27 amino acid Brt-specific sequence. It has been suggested that as a result of this alternative splicing event, Brt resides in the cytoplasm, unlike Rse which is expressed on the cell surface. Ekt-2 also lacks an N-terminal signal peptide which is substituted with a 45 amino acid Ekt-2-specific sequence. Protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.