Product Description
Calnexin Antibody [CANX/1541] | 33-581 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: A partial human recombinant protein was used as the immunogen for the Calnexin antibody.
Research Area: Neuroscience
Tested Application: IF, IHC-P, Flow, WB
Application: WB: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10^6 cells
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 1-2 ug/ml for 30 min at RT
Titering of the Calnexin antibody may be required for optimal performance.
Specificiy: Does not react with rat
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: CANX/1541
Isotype: IgG2b, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER) -associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.