Product Description
CD56 Antibody [CDLA56] | 33-982 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human protein was used as the immunogen for the CD56 antibody.
Research Area: Neuroscience, Stem Cell
Tested Application: Flow, IF, WB, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Titering of the CD56 antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: CDLA56
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: neural cell adhesion molecule, NCAM, antigen recognized by monoclonal 5.1H11, NCAM-1, N-CAM-1, MSK39, CD56, neural cell adhesion molecule 1, NCAM1
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: CD56/NCAM is a cell adhesion protein which is a member of the immunoglobulin superfamily. It is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. CD56 has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. [UniProt]