Product Description
Connexin 32 Antibody [M12.13] | 33-673 | ProSci
Host: Mouse
Reactivity: Rat, Mouse, Human
Homology: N/A
Immunogen: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1) .
Research Area: Membrane, Cancer, Signal Transduction, Neuroscience
Tested Application: Flow, IF, WB, IHC-Fr
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (Frozen) : 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Connexin 32 antibody should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: M12.13
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: GJB1, CMTX, CX32, CMTX1
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.