Product Description
FTO Antibody [FT62-6] | 36-084 | ProSci
Host: Mouse
Reactivity: Mouse
Homology: N/A
Immunogen: Recombinant human FTO.
Research Area: Obesity
Tested Application: E, IHC, IP, WB
Application: ELISA: (direct and indirect: 1:2, 000-1:5, 000) . Immunohistochemistry: (paraffin sections (1:100-1:500) ) . Immunoprecipitation: (5ug/ml per sample in a final reaction volume of 500-1000ul) . Western Blot: (1:2, 000-1:5, 000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Specificiy: Recognizes mouse FTO. Weakly cross-reacts with human FTO. Does not cross-react with rat FTO.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: FT62-6
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.
Alternate Name: Fat Mass and Obesity-associated Protein; FATSO
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.