Product Description
MCAM Antibody [C264] | 99-302 | ProSci
Host: Mouse
Reactivity: Chicken, Turkey
Homology: N/A
Immunogen: Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos
Research Area: Cancer, Immunology, Signal Transduction
Tested Application: IHC-Fr, Flow, IP
Application: MCAM Antibody [C264] for use in flow cytometry, immunocytochemistry, and immunoprecipitation assays.
Specificiy: MCAM
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: [C264]
Isotype: IgG2b
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration: 0.5 mg/mL
Storage Condition: Store vial at 2-8˚C
Alternate Name: CD146, HEMCAM, melanoma cell adhesion molecule
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm’s kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes.