Product Description
NOX4 Antibody [NX2/1524] | 33-763 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Human recombinant protein was used as the immunogen for this NOX4 antibody.
Research Area: Stem Cell
Tested Application: E, IF, WB
Application: IF: 1-2 ug/ml
ELISA: suitable; order BSA-free formulation for coating
WB: 0.5-1 ug/ml
Titering of the NOX4 antibody may be required for optimal performance.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: NOX4/1245
Isotype: IgG2b, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.