Product Description
NS3 Antibody [1B6] | 36-095 | ProSci
Host: Mouse
Reactivity: HCV
Homology: N/A
Immunogen: Recombinant hepatitis C virus (HCV) NS3 serine protease domain.
Research Area: Innate Immunity
Tested Application: ICC, WB
Application: Immunocytochemistry: (paraformaldehyde fixation) (1:50) . Western Blot: (1:500) .
Specificiy: Recognizes HCV NS3 (genotype 1a and 1b) (epitope mapped to NS3 aa 160-193) , recognizes NS3 alone or the NS3-4A complex. Does not cross-react with HCV NS3 (genotype 2a) . t
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: >95% (SDS-PAGE)
Clonality: Monoclonal
Clone: 1B6
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.
Alternate Name: Nonstructural Protein 3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.