Product Description
NS5B Antibody [5B-3B1] | 36-096 | ProSci
Host: Mouse
Reactivity: HCV
Homology: N/A
Immunogen: Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a) .
Research Area: Innate Immunity
Tested Application: WB
Application: Western Blot: (1:1, 000) .
Specificiy: Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382) . It recognizes genotype 1 a and also 1b (albeit with lower affinity) . It does not recognize genotype 2a.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: >95% (SDS-PAGE)
Clonality: Monoclonal
Clone: 5B-3B1
Isotype: IgG2b
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.
Alternate Name: Nonstructural Protein 5B
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.