Product Description
Progesterone Receptor Antibody [PGSR17] | 33-892 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human Progesterone receptor protein was used as the immunogen for this antibody.
Research Area: Cancer, Neuroscience, Signal Transduction
Tested Application: IHC
Application: IHC (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Progesterone receptor antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: PGSR17
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa) . Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.