Product Description
Recombinant EpCAM Antibody [rVU-1D9] | 33-809 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Small cell lung carcinoma cells were used as the immunogen for this recombinant Ep-CAM antibody.
Research Area: Cancer, Cell Cycle, Signal Transduction
Tested Application: IHC-P
Application: Prediluted format: incubate for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Ep-CAM antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: Does not react with rat
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Recombinant Monoclonal
Clone: rVU-1D9
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Epithelial cellular adhesion molecule (EpCAM) , is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. The VU-1D9 antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.