Product Description
TFF1 Antibody [BCEIP1] | 33-997 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: A C-terminus peptide was used as the immunogen for the TFF1 antibody.
Research Area: Signal Transduction
Tested Application: IF, IHC-P
Application: Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 1-2 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Titering of the TFF1 antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: BCEIP1
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, TFF1, BCEI, PS2
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: TFF1/Breast cancer estrogen inducible protein pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. TFF1/pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. TFF1 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract.