Product Description
Villin Antibody [VIL1/1314] | 33-717 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
Research Area: Homeostasis
Tested Application: WB, Flow, IF, IHC-P
Application: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
Western blot: 1-2 ug/ml
IHC (FFPE) : 0.25-0.5 ug/ml for 30 min at RT
Titration of the Villin antibody may be required for optimal performance.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: VIL1/1314
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: VIL1, D2S1471, Villin, Villin 1, Villin-1, VIL
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Recognizes a protein of ~93kDa which is identified as Villin (VIL1) . It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.