Product Description
VLDLR Antibody [VLPR-1] | 34-060 | ProSci
Host: Mouse
Reactivity: Human, Rat
Homology: N/A
Immunogen: A human partial recombinant protein from the C-terminal region was used as the immunogen for this VLDLR antibody.
Research Area: Cancer, Obesity, Neuroscience, Signal Transduction
Tested Application: WB, Flow, IF, IHC
Application: Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
Western blot: 1-2 ug/ml
IHC (FFPE) : 1-2 ug/ml (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the VLDLR antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue is enhanced by boiling sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: VLPR-1
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Very Low Density Lipoprotein Receptor (VLDLR/VLDL receptor) is a member of the LDL receptor gene family which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF) -like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDL receptor is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein) , which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.