Product Description
von Willebrand Factor / vWF Antibody [VWF/1465] | 33-769 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Amino acids 1815-1939 from the human protein were used as the immunogen for this vWF antibody.
Research Area: Cancer, Stem Cell
Tested Application: Flow, IF, IHC-P, WB, IP
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 1-2 ug/ml for 30 minutes at RT
WB: 0.5-1 ug/ml
IP: 1-2ug/500ug lysate
The optimal dilution of the vWF antibody for each application should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: VWF/1465
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.