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Product Description
Flagellin Recombinant Protein | 90-305 | ProSci
Tested Application: N/A
Application: This recombinant proteins is for research use only.
Tpredicted Molecular Weight: N/A
Physical State: Lyophilized
Buffer: Contains PBS. Reconstitute with 100 μl sterile water.
Storage Condition: Stable for at least 6 months after receipt when stored at -20˚C.
Alternate Name: FliC
Background: N/A
Shipping: N/A
Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Source: E. coli
Species: Human, Mouse
By Source: E. Coli
By Species: Human, Mouse
Fusion Tag: His Tag
Sequence: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.
Bological Activity: Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5 ug/ml on bone-marrow macrophages in the presence of streptolysin O (SLO), a protein that allows delivery of exogenous molecules into the cytosol of living cells.
Purity: >95% (SDS-PAGE).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
