Product Description
POU4F3 Antibody | 27-780 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat, Dog, Zebrafish
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU4F3.
Research Area: Transcription, Neuroscience
Tested Application: E, WB, IHC
Application: POU4F3 antibody can be used for detection of POU4F3 by ELISA at 1:312500. POU4F3 antibody can be used for detection of POU4F3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 37 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein A chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU4F3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: POU4F3, BRN3C, DFNA15
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: POU4F3 is capable of activating both BDNF and NT-3 promoters in inner ear sensory epithelial cell lines. Mutant POU4F3 loses most of its transcriptional activity and most of its ability to bind to DNA. The mutation causes autosomal-dominant nonsyndromic hearing loss and eventually leads to hair cell morbidity in affected family members.