Product Description
N-RAS Antibody | 7381 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%) , Guinea pig: (93%) , Chicken: (80%)
Immunogen: N-RAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human N-RAS.
The immunogen is located within the last 50 amino acids of N-RAS.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB
Application: N-RAS antibody can be used for detection of N-RAS by Western blot at 1 and 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificiy: N-RAS antibody is predicted to not cross-react with H-RAS
Positive Control 1: Cat. No. 1202 – A431 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 22 kDa
Observed: 22 kDa
Validation: N/A
Isoform: N/A
Purification: N-RAS Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: N-RAS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: N-RAS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: N-RAS Antibody: NS6, ALPS4, N-ras, NRAS1, HRAS1, GTPase NRas, Transforming protein N-Ras
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.