Product Description
RAET1E Antibody | 8193 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: RAET1E antibody was raised against an 18 amino acid peptide near the center of human RAET1E.
The immunogen is located within amino acids 160 - 210 of RAET1E.
Research Area: Innate Immunity, Cancer
Tested Application: E, WB, ICC
Application: RAET1E antibody can be used for detection of RAET1E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificiy: RAET1E antibody is human and mouse reactive. At least four isoforms of RAET1E are known to exist; this antibody will detect all four.
Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 29 kDa
Observed: 29 kDa
Validation: N/A
Isoform: N/A
Purification: RAET1E antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: RAET1E antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: RAET1E antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Retinoic acid early transcript 1E, NKG2D ligand 4, N2DL-4, NKG2DL42, RL-4, ULBP4, LETAL
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The Retinoic acid early transcript 1E (RAET1E) belongs to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3 (1) . Like the related protein RAET1G, RAET1E differs from other RAET1 proteins in that they have type I membrane-spanning sequences at their carboxy termini rather than glycosylphosphatidylinositol anchor sequences (2) . RAET1E functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses (1, 3) .