Product Description
TYW2 Antibody | 6375 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (74%)
Immunogen: TYW2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2.
The immunogen is located within the first 50 amino acids of TYW2.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB, ICC
Application: TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificiy: TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms
Positive Control 1: Cat. No. 1204 - K562 Cell Lysate
Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: TYW2 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TYW2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TYW2 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TYW2 Antibody: TYW2, TRM12, TYW2, tRNA wybutosine-synthesizing protein 2 homolog, Alpha-amino-alpha-carboxypropyl transferase TYW2, tRNA-yW-synthesizing protein 2
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TYW2 Antibody: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.