Product Description
TRIM30 Antibody | 4747 | ProSci
Host: Rabbit
Reactivity: Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Human: (80%)
Immunogen: TRIM30 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of the mouse TRIM30.
The immunogen is located within amino acids 110 - 160 of TRIM30.
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: TRIM30 antibody can be used for detection of TRIM30 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 107 kDa
Observed: 105 kDa
Validation: N/A
Isoform: N/A
Purification: TRIM30 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TRIM30 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TRIM30 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TRIM30 Antibody: Rpt1, Rpt-1, Trim30, Rpt1, Tripartite motif-containing protein 30A, Down regulatory protein of interleukin-2 receptor
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TRIM30 Antibody: TRIM30 belongs to a family of the tripartite motif (TRIM) proteins involved in the regulation of cell proliferation, differentiation, development, oncogenesis, apoptosis and antiviral responses. The TRIM protein family is an expanding family of RING ('really interesting new gene') proteins, also known as RBCC proteins as they contain an RBCC motif, which comprises a RING domain, one or two B-boxes and a predicted coiled-coil region. Studies have shown that some TRIM family members are critical to innate immunity; TRIM5, TRIM19 and TRIM25, for example, have been shown to restrict viral infection. A recent study shows that TRIM30 functions as a negative modulator of the TLR signaling pathway, by targeting TAB2 and TAB3, and contributes to the inhibition of TLR-mediated NF-κB activation. The importance of TRIM30 in the attenuation or termination of NF-κB activation suggests that targeting of TAB2 and TAB3 by TRIM30alpha may be a mechanism for modulating many types of immune responses.