Product Description
CLCNKB Antibody | 28-218 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLCNKB.
Research Area: Membrane
Tested Application: E, WB
Application: CLCNKB antibody can be used for detection of CLCNKB by ELISA at 1:312500. CLCNKB antibody can be used for detection of CLCNKB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 76 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by peptide affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLCNKB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: CLCNKB, CLCKB, ClC-K2, ClC-Kb
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Chloride channel Kb (CLCNKB) is a member of the CLC family of voltage-gated chloride channels, which comprises at least 9 mammalian chloride channels. Each is believed to have 12 transmembrane domains and intracellular N and C termini. Mutations in CLCNKB result in the autosomal recessive Type III Bartter Syndrome. CLCNKA and CLCNKB are closely related (94% sequence identity) , tightly linked (separated by 11 kb of genomic sequence) and are both expressed in mammalian kidney.