Product Description
Recombinant Basic Cytokeratin Antibody [KRTH/1576R] | 33-833 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Research Area: Signal Transduction
Tested Application: Flow, IF, WB, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein A affinity chromatography
Clonality: Recombinant Monoclonal
Clone: KRTH/1576R
Isotype: IgG, k
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: KRT2B, KRT2P, Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, CK-2P, HUMCYT2A
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.