Product Description
Recombinant Glycophorin A Antibody [GYPA/1725R] | 34-089 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human GPA protein was used as the immunogen for the recombinant Glycophorin A antibody.
Research Area: Homeostasis
Tested Application: IF, IHC-P, Flow
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Optimal dilution of the recombinant Glycophorin A antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein A affinity chromatography
Clonality: Recombinant Monoclonal
Clone: GYPA/1725R
Isotype: IgG, k
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: GYPA, glycophorin A (includes MN blood group) , HGNC:4702, GPA, GPErik, GPSAT, HGpMiV, HGpMiX, HGpMiXI, HGpSta (C) , MN, MNS, Mi.V glycoprotein (24 AA) , erythroid-lineage-specific membrane sialoglycoprotein, glycophorin A, glycophorin Er
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175) ; binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV) . [UniProt]