Product Description
RFT1 Antibody | 26-488 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFT1.
Research Area: Membrane, Cell Cycle, Signal Transduction
Tested Application: E, WB
Application: RFT1 antibody can be used for detection of RFT1 by ELISA at 1:1562500. RFT1 antibody can be used for detection of RFT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 60 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by peptide affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store RFT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: RFT1, CDG1N
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a¡¡Man (5) GlcNAc (2) -dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5) GlcNAc (2) -PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man (5) GlcNAc (2) -dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5) GlcNAc (2) -PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]) .