Product Description
SMAD6 Antibody | 27-669 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat, Dog
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD6.
Research Area: Transcription, Signal Transduction
Tested Application: E, WB, IHC
Application: SMAD6 antibody can be used for detection of SMAD6 by ELISA at 1:62500. SMAD6 antibody can be used for detection of SMAD6 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 53 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is supplied as total IgG.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: SMAD6, AOVD2, MADH6, MADH7, HsT17432
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.