Product Description
MIP-2 Antibody (biotin) | XP-5149Bt | ProSci
Host: Rabbit
Reactivity: Rat
Homology: N/A
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat GRO/β/MIP-2 (rat GRO/β/MIP-2) .
Research Area: Chemokines & Cytokines, Signal Transduction, Homeostasis, Antibody Pairs
Tested Application: E, WB
Application: ELISA:
Sandwich:
To detect Rat GRO-β/MIP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat GRO-β/MIP-2 (XP-5149) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GRO-β/MIP-2.
Western Blot:
To detect rat GRO/β/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat GRO/β/MIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Anti-rat GRO/β/MIP-2 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Biotin
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: GROB/MIP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: Mip-2, Scyb2, Cinc3, Mip-2, Mip2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, CINC-3
User Note: Centrifuge vial prior to opening.
BACKGROUND: Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta.